A COMPARATIVE STUDY OF ANGIOTENSIN CONVERTING ENZYME (ACE) GENE POLYMORPHISM OF EASTERN INDIAN BENGALI AND WESTERN INDIAN MEWARI TYPE 2 DIABETES PATIENTS IN INDIA
Abstract
Author(s): Pulakes Purkait*, Kalpataru Halder; Abhishikta Ghosh Roy; P N. Venugopal; B.N. Sarkar; J.M. Naidu
Diabetes mellitus is a rapidly emerging global health problem and it is expected to cross the pandemic level within a span of another two decades. World-wide its frequency is expected to rise from 17 1 million to 366 million within the next two decades and India alone will take almost 25% shares from it to become the leading country with highest number of diabetic patients. For the present study 111 type 2 diabetes (59 Bangali and 52 Mewari) were enrolled. Anthropometric measurement and Clinical data- including information on duration of diabetes, presence of any complication, history of other disorders, systolic and diastolic blood pressure- were collected. The weight (kg) and height (cm) were recorded, the body mass index (BMI) was calculated and Genomic DNA was isolated from 5ml of peripheral blood samples of 111 type 2 diabetes individuals. Further, PCR has been performed to know the frequency of ACE I/D polymorphism. Our study reveals that anthropometric measurements and few bio-chemical parameters were significantly varying in type 2 diabetes patients among western Indian Mewari population and eastern Indian Bengali population. Distribution of ACE genotypes as well as their allele frequency doesn’t differ significantly. It can presume that ACE gene I/D polymorphism, an Alu insertion polymorphism, can be used as a suitable marker for studying genetic variation among different human populations because of its stable nature as well as representing a unique evolutionary event. Key words: Type 2 Diabetes, ACE polymorphism, Anthropometry, Bengali, Mewari
