DEVELOPMENT AND VALIDATION OF STABILITY INDICATING UPLC METHOD FOR SIMULTANEOUS QUANTIFICATION OF IMIDACLOPRID, THIRAM AND CARBOXIN IN PESTICIDE FORMULATION
Abstract
Author(s): Dilip K. Patel*, Babu Lal Swami, T.L. Rajawat
A novel stability-indicating ultra-performance liquid chromatography (UPLC) method has been developed and validated for quantification of Imidacloprid, Thiram and carboxin in pesticide formulation (FS ), using Poroshell 120 EC-C18 (100 mm × 4.6 mm, 2.7µm) column. Mixture of Water: Methanol (40:60 v/v) was used as mobile phase. The flow rate was kept 0.40 ml/min and detection was carried out at 250 nm. The limit of detection was 0.0006mg/ml, 0.0007mg/ml and 0.0007mg/ml for Imidacloprid, Thiram and Carboxin respectively. The limit of quantitation values was 0.0019mg/ml, 0.0015mg/ml and 0.0015mg/ml for Imidacloprid, Thiram and Carboxin respectively. The linearity of proposed method was investigated in the range of 0.0019-0.596mg/ml (r2=0.9997), 0.0015-0.178mg/ml (r2=0.9997) and 0.0015-0.175mg/ml (r2=0.9997) for Imidacloprid, Thiram and Carboxin respectively. The percentage recovery found to be in range from 98.1-99.9 %, 98.3-100.7% and 98.6-99.6% for Imidacloprid, Thiram and Carboxin respectively. The % RSD values for intraday precision study and interday precision study were <1.66, <2.0 and <2.0 for Imidacloprid, Thiram and Carboxin respectively, as per modified Horwitz equation as requirements by CIPAC. The method was found to be specific, linear, precise, accurate and robust. This method is also useful for quantification of Imidacloprid, Thiram and Carboxin in their single or combination formulated products, with different strengths and different formulation types.
