HPTLC FINGER PRINTING ANALYSIS OF THE PDM EXTRACT SCOPARIA DULCIS LINN.
Abstract
Author(s): Jitendra Bajaj*, Neeraj Upmanyu
Scoparia dulcis linn (family: Scrophulariaceae) is a glabrous under shrub with small white flowers commonly found on the waste lands and fallow fields. This plant was widely used in the indigenous system of medicine. Infusion of leaves is used in fever, cough, and bronchitis and as gargle for toothache. The PDM extract, active fractions and pure compounds were subjected to HPTLC analysis (CAMAG, Muttenz, Switzerland) using precoated TLC plate of silica gel 60 F254 (Merck, Darmstadt, Germany). 5 mg/ml solution of PDM extract was spotted using Linomat IV automatic spotter. TLC was developed using chloroform methanol (9:1) as solvent system. After development, TLC plate was dried and scanned using HPTLC analyzer and the peaks were recorded at wavelength of 254 nm and 366 nm. The plats were also sprayed with anisaldehyde sulphuric acid spraying reagent.
