HYPERGLYCEMIA RELATED OXIDATIVE STRESS: BIOMARKERS IN TYPE 1 AND2 DIABETES MELLITUS
Abstract
Author(s): Dr. George Gborienemi, S., Oyindo, Charles, Dienize George,
The production of reactive oxygen species resulting in oxidative stress due to formation of free radicals is now known to be a feature of many diseases including diabetes mellitus. In this study, we have used some biomarkers of oxidative stress, TBARs, SOD, GSH-Px, HbA1c, glyoxal and methylglyoxal along with some other parameters, glucose and insulin to objectively estimate and analyse the condition as indicators in the control (normal) subjects and in patients with pathologic disease process of type 1 and 2 diabetes. Applying kinetic, spectrophotometric, radioimmunoassay and chromatographic methods we have assayed the indicator biomarkers. Results obtained demonstrate the fact that higher increases in the concentration of the analytes up to 3-fold were found in type 2 diabetics in the concentration of glyoxal, methylglyoxal, TBARs, HbA1c at p=0.005, p>0.05, p=0.002 and p=0.003 respectively when compared with the control. There were, however a moderate decrease in the concentration of S0D and GSH-Px. We summaries that a combination therapy for the oxidative stress management to reduce TBARS, HbA1c and the dicarbonyls can inhibit further complications in diabetics. Keywords: Biomarkers, hyperglycemia, oxidative stress, Type 1, Type 2 Diabetes.
