ONE-FACTOR-AT-A-TIME (OFAT) OPTIMIZATION OF ?-GALACTOSIDASE PRODUCTION FROM ASPERGILLUS FLAVUS GR. NFCCI-2728 IN SUBMERGED FERMENTATION.
Abstract
Author(s): J. D. Patel*, B. V. Raol, N. H. Bhavsar and G. G. Raol*
Production of ß-galactosidase enzyme under submerged fermentation was carried out using Aspergillus flavus gr. NFCCI-2728. Optimization of different cultural conditions viz., incubation time, nitrogen source and its concentration, carbon source and its concentration, pH and inoculum size were carried out using one factor at time approach (OFAT) in Czapek’s-Lactose (CL) Medium for enhanced production of ß-galactosidase enzyme. The highest yield of ß-galactosidase was achieved 843.75 Umg-1 which was 4.75 times higher than unoptimized medium
