SOMOCLONAL VARIATION STUDIES ON CASSIA OCCIDENTALIS LINN USING SDS-PAGE
Abstract
Author(s): Johnson M* and Narayani M
The present study was aimed to reveal the somoclonal variation in Cassia occidentalis Linn using SDS- PAGE as a tool. For the electrophoresis studies, mother plant and leaves segments derived calli and nodal segment derived plants young leaves were harvested and ground on ice cold mortar and pestle with 0.1 M phosphate buffer (pH 7.0). The SDS- PAGE gel electrophoresis was performed by Anbalagan method. The protein banding profile showed the different banding profile in leaves segments derived calli. The mother plants and nodal segments derived plantlets showed the similar banding pattern in the gel system. The SDS-PAGE protein banding patterns of the somoclonal variant is used as a biochemical marker for the future plant breeding or genetic improvement programme.
