ANTIMICROBIAL EVALUATION AND DETERMINATION OF TOTAL PHENOLIC CONTENT OF MELIA AZEDARACH
Abstract
Author(s): Biresh Kumar Sarkar, Sunil Kumar Punia, Monoj Kumar Gupta, Shashimindar Yadav, Prashant Soni
Melia azedarach used traditionally for various diseases and infections as per the klowledge of ayurveda. The acetone extraction was carried out of the plant. The phytochemical groups were identified by the tests of characterization and then quantified by the test of total phenolics. Total phenolics were determined using Folin–Ciocalteu reagent procedure. The total polyphenolic content and flavonoid content in the extract were found to be 71.13±0.11 mg/g and 11.14±0.12 mg/g respectively; since antimicrobial activity is related to phenolic compounds contained thus extract was also evaluated for the antibacterial activity. Result of study suggested that Melia azedarach having great potential as antibacterial agent. The extract at dose level of 200 mcg/ml showed highest inhibition against P. aerugunosa, S. aureus and E. coli.
