Author(s): Shaza Wagiealla Shantier, Elrashied Ali Elobaid Garelnabi, Elrasheed Ahmed Gadkariem
Simple, sensitive and accurate derivative spectrophotometric and HPLC methods were developed for the determination of niclosamide in bulk and dosage forms. The spectrophotometric method was based on the measurement of the first derivative spectrum for the methanolic solution of niclosamide at ?max 351nm. The HPLC-separation was conducted on Shimpac C-18 (25x4.6mm) column using a mobile phase consisted of 70: 30 v/v methanols: water. System suitability was assessed by measurement of factors affecting column efficiency. Beer’s law was obeyed over the concentration range 2-12µg/ml with a correlation coefficient not less than 0.999. The added recovery results were 100.80 ± 0.59 (n=3), which indicates the absence of interference by the tablets excipients. The results obtained by the developed methods for the tablet dosage form were statistically compared with those of a reported method and evaluated at 95% confidence limits.
Journal of Harmonized Research in Pharmacy received 119 citations as per google scholar report