Author(s): Monesh Likhitkar*and Milind Pande
We previously demonstrated that stress induce alopecia in mice. This effect was prevented by the oral administration of Pueraria tuberosa alcoholic extract, an isoflavone and phytosterol e containing plants which prevent hair loss alopecia condition. Objectives: The present study aimed at hair growth activity of Pueraria tuberosa by Using Stress Induced Stress induced alopecia and modulating the mechanisms responsible for this condition. Methods: 4 Group of mice were exposed whole-body to sonic stress. Groups of mice received the Pueraria tuberosa extract and standard Minoxidile 5% solution administered orally to elucidate the biological activity in vivo. Occurrence of alopecia was evaluated for up to 21 days of exposure, and the hair density, hair population, lymphocyte count and testosterone level in peripheral blood was investigated. Results: The hair density and hair population decrease and lymphocyte count was increased by stress; irrespective of treatment with ultrasonic sound. Test drug extract show decreases the lymphocyte count as compared to increased count in toxic group. Conclusions: Decrease in lymphocyte count in case of stress is a good sign of preventing hair loss due to the presence of isoflavonoid , protein and amino acid, insulin growth factor and may be due to increase blood circulation which promote hair growth in humans. Keywords: Isoflavonoid, Phytosterole, Pueraria tuberosa, Lymphocyte count, Hair density.