Author(s): Manoj Kumar Kamshetti, Sunita Jhade, Pushpendra Sharma, Deenanath Jhade
Background and Objectives: Garcinia cambogia (Guttiferae), generally known as Malabar tamarind is a dried fruit rind commonly found in Western ghats of south India. It extends from Konkan southward to Travancore and in the forests of Nilgiris. It is highly regarded for their medicinal properties in the indigenous systems of medicine. The present study deals with the isolation and characterization of the chemical constituents of Garcinia cambogia. Methodology: Authenticated aqueous extract of Garcinia cambogia is collected from Pharmed Medicare, Bangalore. Phytochemical screening of extract has been done. Isolation of phytoconstituents by column chromatography and/or alternate method of isolation was carried out with aqueous extract. The isolated compound was identified by comparing the HPLC fingerprint of the sample with the standard chromatogram of (-)-HCA. Results: One compound has been isolated and estimated as (-)-Hydroxy Citric Acid by comparing the HPLC data of sample and standard. Conclusion: The isolation procedure used is suitable to isolate the marker constituent from aq extract of Garcinia cambogia. Keywords: Garcinia cambogia, (-)-Hydroxy Citric Acid, HPLC.