Journal of Harmonized Research in Pharmacy

ISOLATION AND INVESTIGATION OF ANTIMICROBIAL ACTIVITY OF THE LEAVES OF THEA SINENSIS

Abstract

Author(s): Jiten Mishra*, Sanjay Kumar Nayak and Shudhir Kumar Sahoo

In the present study the ethanolic extract and isolated caffeine obtained from the Thea sinesis were tested for antibacterial activity against 13 Bacterial strains both gram positive and gram negativ e by determining minimum inhibitory concentration (MIC) and zone of inhibition (ZOI). MIC values were compared with standard chloramphenicol in concentrations of 30 & 100 µg/ml. The result of MIC & ZOI and its competition with standard antibiotics it evident that the ethanolic extract is active against both gram positive and gram negative bacteria. Further the isolated caffeine was characterized by different analytical techniques like TLC, UV-VIS spectroscopy & HPLC and compared with the slandered.